Research Interests 【 display / non-display 】

Research Interests 【 display / non-display 】

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Research Areas 【 display / non-display 】

Research Areas 【 display / non-display 】

Papers 【 display / non-display 】

Papers 【 display / non-display 】

• Development of RT h-CLAT, a Rapid Assessment Method for Skin Sensitizers Using THP-1 Cells as a Biosensor

  Hiroki Koyama, Ayami Maeda, Peiqi Zhai, Keiichiro Koiwai, Kouichi Kurose , 2024.12

  Biosensors

  DOI

• A highly sensitive detection of salmonids by real-time PCR targeting salmonid-specific retrotransposon Hpa I element

  Wei Cui, Yuya Negoro, Hiroki Koyama, Kouichi Kurose , 2024

  Fisheries Science

  DOI

• Rapid and specific detection of mackerels of the genus Scomber using loop-mediated isothermal amplification (LAMP)

  Wei Cui, Hiroki Koyama, Kouichi Kurose , 2024

  Fisheries Science

  DOI

• A newly identified enzyme from Japanese common squid Todarodes pacificus has the ability to biosynthesize d-aspartate.

  Hiroki Koyama, Yui Takahashi, San Matori, Hisato Kuniyoshi, Kouichi Kurose , 2023.11

  Archives of biochemistry and biophysics

  DOI

• Comprehensive and species-specific detection of mackerels of the genus Scomber in processed foods using SYBR Green-based real-time PCR

  Wei Cui, Yuki Sano, Hiroki Koyama, Kouichi Kurose , 2023.10

  Fisheries Science

  DOI

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Books 【 display / non-display 】

Books 【 display / non-display 】

• 食品衛生学　第3版

  黒瀬光一、本城賢一、宮本敬久 , 2024.07

  東京化学同人 , p93～138

• 食品衛生学　第２版

  一色賢司、小田隆弘、黒瀬光一、宮本敬久 , 2019.03

  東京化学同人 , p90～100、p101～131

Grant-in-Aid for Scientific Research 【 display / non-display 】

Grant-in-Aid for Scientific Research 【 display / non-display 】

• カブトガニを救う新規エンドトキシン試験法の開発

  Project Period (FY)： 2024/04  -  2027/03  Investigator(s): 黒瀬光一

  Grant-in-Aid for Scientific Research(C)  Principal Investigator  24K05495 

• Development of a novel in vitro skin sensitization assay with predictive ability comparable to animal studies

  Project Period (FY)： 2021/04  -  2024/03  Investigator(s): 黒瀬光一

  Grant-in-Aid for Scientific Research(C)  Principal Investigator  21K02070 

• Development of detection method for potential food allergens

  Project Period (FY)： 2018/04  -  2021/03  Investigator(s): Kurose Kouichi

  Grant-in-Aid for Scientific Research(C)  Principal Investigator  18K02220 

  We attempted to differentiate THP-1, a human monocyte-like cell line, into cells that are responsive to allergens and capable of proliferating, and conducted various cell-based experiments to development an allergenicity evaluation system of food protein by using the induction of allergen-specific gene expression of some genes as an indicator. But in conclusion, we were unable to establish such an allergenicity evaluation system.
  On the other hand, it was found that one of the induction of gene expression, which was used as an indicator of allergenicity in the course of this study, can be used as an indicator to evaluate endotoxin activity in a simple and highly sensitive manner. Since extremely small amounts of endotoxin in the bloodstream can cause fever and shock, strict control of endotoxin is required for injectable drugs, therefore our new endotoxin test method would be worth developing.

• Utilaization of isopod as a foodstuff

  Project Period (FY)： 2016/04  -  2019/03  Investigator(s): Kazufumi Osako

  Grant-in-Aid for Scientific Research(C)  Co-Investigator  16K07869 

  To utilize isopod Bathynomus doederleinii as a foodstuff, recovering method of protein from isopod, gel-forming ability of recovered protein, endogenous protease of recovered protein, and allergen were investigated. The protein with relatively higher functionality could be obtained by salt water treatment method. Although the gel-forming ability of recovered protein was somewhat lower than that of fish meat, recovered meat from isopod was assumed to be utilized as a raw material of kamaboko. The somewhat lower gel-forming ability is possibly caused by metallo-type protease and serine-type protease. However, isopod was clarified to contain tropomyosin which is an allergen of crustaceans. The further study is needed to reduce the amount of allergen including tropomyosin.

• 食物アレルゲンに対する新規評価系の構築

  Project Period (FY)： 2015/04  -  2018/03  Investigator(s): 黒瀬光一

  Grant-in-Aid for Scientific Research(C)  Principal Investigator  15K00812 

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Lesson Subject 【 display / non-display 】

Lesson Subject 【 display / non-display 】