Research Interests 【 display / non-display 】

Research Interests 【 display / non-display 】

Research Areas 【 display / non-display 】

Research Areas 【 display / non-display 】

Papers 【 display / non-display 】

Papers 【 display / non-display 】

• Real-time glucose monitoring biosensor system assesses the effects of different environmental light colors on Nile tilapia stress response

  Tengyu Liu, Haiyun Wu, Masataka Murata, Haruto Matsumoto, Hitoshi Ohnuki & Hideaki Endo , 2024.06

  Fisheries Science

  DOI

• Expanding Applicability of Wireless Biosensor System for Monitoring Fish Stress Response through Abdominal Interstitial Fluid

  Chihiro Morita,Haiyun Wu,Atsuki Takizawa,Masataka Murata, Haruto Matsumoto, Hitoshi Ohnuki, Hideaki Endo , 2023.08

  Sensors and Materials

  DOI

• Modification of the oxygen radical absorbance capacity assay and its application in evaluating the total antioxidative state in fish

  T. Nakano, S. Hayashi, Y. Ochiai, H. Shirakawa, H. Wu, H. Endo, H. Yu , 2022.10

  Advances in Redox Research

  DOI

• A novel interactive biosensor system for real-time remote stress response monitoring and visualization by using bi-directional data link

  H. Wu, K. Yamada, M. Murata, H. Matsumoto, H. Ohnuki, H. Endo , 2022.05

  Biosens. Bioelecton.:X

  DOI

  OACIS Paper URL

• Toward a Practical Impedimetric Biosensor: A Micro-Gap Parallel Plate Electrode Structure That Suppresses Unexpected Device-to-Device Variations

  H. Honda, Y. Kusaka, H. Wu, H. Endo, D. Tsuya, H. Ohnuki , 2022.04

  ACS Omega

  DOI

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Books 【 display / non-display 】

Books 【 display / non-display 】

• テレワーク社会を支えるリモートセンシング

  遠藤英明, 呉 海云 , 2021.04

  シーエムシー出版 , 第18章 魚の体調センシング , 0-0

• 酵素トランスデューサーと酵素技術展開～ウエアラブルIoTのためのリアルタイム酵素センシング編

  遠藤英明，呉 海云 , 2020

  シーエムシー出版書籍 , バイオセンサによる魚類のストレス応答の可視化 , 44-51

• FOOD BIOSENSORS

  H. Wu and H. Endo , 2016.03

  Royal Society of Chemistry , Biosensor systems for the monitoring of fish health and freshness in aquaculture (Chapter 18) , 414-431

• バイオセンサの先端科学技術と新製品への応用開発，技術情報協会編

  遠藤英明 , 2014.03

  技術情報協会 , 第9章第9節「バイオセンサによる迅速・簡便な魚類の健康診断～さかなドックの創出に向けて～」 , 377-382

• 生物学辞典

  石川統 他　編 , 2010.05

  東京化学同仁 , 用語解説のため無し , 1006p, 1008p

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Grant-in-Aid for Scientific Research 【 display / non-display 】

Grant-in-Aid for Scientific Research 【 display / non-display 】

• Development of novel smart biosensing system for fish

  Project Period (FY)： 2021/04  -  2025/03  Investigator(s): 遠藤英明

  Grant-in-Aid for Scientific Research(B)  Principal Investigator  23K21235 

• Development of biosensor system for fish physiological state by using bidirectional communication technology

  Project Period (FY)： 2017/04  -  2021/03  Investigator(s): Endo Hideaki

  Grant-in-Aid for Scientific Research(B)  Principal Investigator  17H03871 

  The aim of this research was to develop a novel bidirectional wireless biosensing system for easily and intuitively monitoring the physiological state of fish. The system allows for direct visual judgment of different states and real-time monitoring of fish stress according to the commands sent by the client. We first developed a biosensor and a bidirectional communication system for monitoring the physiologic state of fish, such as the stress response, and attached them to multiple test fish. Next, we tried to identify specific individuals swimming in the water by controlling the LED pattern remotely. The fish stress states could also be visualized automatically by changing the color of the LED (Red ⇔ Yellow ⇔ Green) through 2 different preset thresholds. The proposed biosensor system fulfilled the objectives of the research.

• Exploration of fish eustress / distress using wireless biosensor system

  Project Period (FY)： 2016/04  -  2019/03  Investigator(s): Hideaki Endo

  Grant-in-Aid for challenging Exploratory Research  Principal Investigator  16K14969 

  We aimed to clarify the relationship between beneficial stress (eustress) and harmful stress (distress) in fish using a biosensor system to evaluate the stress response. We first developed a biosensor system suitable for our purposes. Next, the test fish (Nile tilapia) were bred with lights of different wavelengths (white, blue, red, or green), and recovery from stress was monitored over time. Breeding under green wavelengths tended to be most effective for recovery. Also, we examined the effect of the color of the inner wall of the fish tank (blue, red, or green) on recovery from stress. The pattern of recovery was also monitored which similar to that under different wavelengths. The red inner wall showed relatively effective for recovery from stress in the test fish compared to the rest color. These findings may be associated with eustress, but differences in the response among individuals were also observed.

• An optical communication-type biosensing system for detecting fish stress

  Project Period (FY)： 2014/04  -  2017/03  Investigator(s): Endo Hideaki

  Grant-in-Aid for Scientific Research(B)  Principal Investigator  26292114 

  We developed a novel biosensing system to monitor fish stress using optical technology. Biosensors to monitor indicators of fish stress such as cortisol, glucose, and cholesterol concentrations were prepared. An immunosensor for determining the cortisol concentration was developed using an anti-cortisol antibody, carbon nanotubes, and an Au electrode. An enzyme sensor for simultaneous monitoring of glucose and cholesterol concentrations was also prepared using immobilized enzyme carbon nanotubes and micro-electrode. We then developed a novel wireless system using optical technology utilizing a light-emitting diode as the transmission device, which was attached to the body of the fish, and glucose levels of stress indicator were monitored in the fish under free-swimming conditions. Successful real-time monitoring of fish stress indicated that the new biosensing system worked well and is a promising tool for monitoring fish health.

• Development of highly sensitive affinity biosensor with interdigitated microelectrodes

  Project Period (FY)： 2013/04  -  2016/03  Investigator(s): OHNUKI Hitoshi

  Grant-in-Aid for Scientific Research(C)  Co-Investigator  25390050 

  Affinity biosensor based on electrochemical impedance spectroscopy possesses attractive advantages. But the small signal intensity limits the detection performance. In this study, we examined several attempts to increase the intensity using the interdigitated microelectrodes.
  First, we intended to optimize the distribution of electric field on the electrode. The edge lines of interdigitated electrodes were covered with SiO2 layer to locate the electric field on the central area of electrode. However, the sensing performance was not as good as normal ones. The result suggested that a disordered surface may increase the performance. To examine this idea, we employed a mixed SAM. One of the SAM molecule has a short and inactive terminal group, and the domains act as pin-hole defects. It was found that the biosensor contains the 75 % areal pin-holes significantly improved the sensing performance. We proposed a model to increase sensing performance by the pin-holes.

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Lesson Subject 【 display / non-display 】

Lesson Subject 【 display / non-display 】